Diluted or Pale Colors

  • "Dilution" Loci
  • This webpage is part of a series on Dog Coat Color Genetics and was last updated on 28 July 2020 by Sheila Schmutz, when it was moved to a different server.

    Please note that the photos on this webpage are from dogs that were actually genotyped using DNA and therefore are verified to have the alleles that they are shown to represent.


    Dilution of Eumelanin and/or Phaeomelanin Caused by MLPH Mutations

    Blue

    Blue or charcoal grey, as a dilution of black, was shown to be caused by the melanophilin gene (MLPH). The blue caused by this gene could be termed "born blue" since it is present from birth. The Blue Doberman is a dog that has the area on its upper torso diluted to slate grey, instead of black, and its tan undersides diluted to a paler shade of tan. The dilution of the tan is much less noticeable than the dilution of black, both both eumelanin and phaeomelanin are affected in dogs of d/d genotype.

    There are now at least three mutations in the MLPH gene that cause dilute coat colors in various breeds: c-22G>A (in noncoding exon 1), c.705G>C, c.106C>T (in exon 2). Not all laborations test for all mutations. One could call these d1. d2, d3. Any two of these mutations in a dog would typically lead to a dilute coat color.

    Blue is the name of a coat color used in breeds like the Great Dane to specify a eumelanin pigmented dog that is diluted to a blue grey. The litter at the left shows blue and black pups in the same litter.

    Blue is also used to describe a coat color in the Chow, as shown by Shadow on the right, and in the Shar-Pei. It also occurs occasionally in Newfoundlands, Large Munsterlanders, Australian Shepherds, Doberman Pinschers, and other breeds. Hence it occurs in a wide variety of breeds and may be quite ancient in origin.

    The Weimaraner is an example of a brown dog with a diluted coat color. This dog is most commonly seen as the short-haired variety on the right, but there is also a long-haired variety shown below. Although Little refered to the Weimaraner as diluted brown, most people call this dog the "grey ghost". Most Weimaraners are b/b at the brown locus and therefore Little was right again! He also referred to them as "d/d" at the dilute locus. Some slate grey dogs resulted from breeding a Weimaraner colored dog to a black dog occur, suggesting this diluter gene is capable of diluting both brown to the Weimaraner pale brown color and black to a slate grey. Little called this color blue in a Great Dane/Pointer crossbred pup.

    Photo by Linda Heeg

    The photo at the right shows that MLPH is able to dilute both black and brown eumelanin pigments in these Labrador Retriever pups..

    In dogs such as the Australian Shepherd, dilution causes black-and-tan dogs to appear charcoal-and-tan. The tan is not affected as dramatically as the charcoal. Kodak is charcoal-and-tan and Peggy is blue merle with tan. The merle seems to make her tan seem even paler than his tan! Notice the marked difference in their nose color however. His is charcoal compared to her pitch black. (Photo by Jonelle Kunkle)

    Italian Greyhounds occur in "blue" and also "blue fawn". Nelson is a good example of a dog that has his black mask diluted to grey and his fawn body diluted to a paler fawn. The Italian Greyhound Club of America provided funding to study IGs that have dilute coat colors with and without hair loss or skin problems. We have already determined that not all dilute IGs have the same MLPH mutation. Note that not all commercial laboratories test for the same mutation.

    Some blue dogs appear to be more prone to skin problems and hair loss, and perhaps skin allergies than dogs that are black. Some breeds have more problems in "born blue" dogs than others. Thus far, it's not clear why.

    The hair loss and skin problems which occurs in blue dogs is called "Black Hair Follicular Dysplasia" in some breeds and "Color Dilution Alopecia" in other breeds. In the past it may sometimes have been called "Blue Dog Syndrome" also.

    DNA Tests for d Allele

    HealthGene offers tests for the common "d" allele. In some breeds such as Large Munsterlanders, Newfoundlands, and Great Danes, this appears to be the only allele causing grey/blue. In other breeds such as Doberman Pinschers, Italian Greyhounds, Chow Chows and Shar-Pei the test could detect carriers of this allele also, but this is not the only allele that causes grey/blue.


    "Blue" Used for other Color Phenotypes

    In other breeds, such as the English Setter, a blue belton means the dog is black and white giving a bluish appearance from a distance. In the case of the "blue belton", no dilution is involved. That phenotype is an affect of the roan, ticking and/or spotting series. Blue in the Blue Heeler is likely a description for roan. (See spotting page for other examples.) Blue merle is the term used for merle dogs with black and grey and white hairs in the marbled or merle pattern as illustrated by Peggy, the "Aussie" above. (See merle page for further information.)


    I Locus, MFSD12

    Phaeomelanin Dilution

    The photo above dramatically illustrates the effect of a co-dominant phaeomelanin diluter gene. The hair clippings are from Toy poodle ears: red, apricot, and cream. Their dam was a black that was not diluted to silver, although she was not pitch black either. She must carry some dilution allele to have a cream pup.

    Sponenberg and Rothschild describe a gene for Intense that dilutes only phaeomelanin, based on the work of Iljin (1941) and later Carver (1984) Diluted phaeomelanin colors are sometimes called cream, buff, apricot, lemon, etc. Hédan and colleagues discovered that this gene is MFSD12 in 2019. They used cream dogs initially and then expanded their study to include cream dogs with black masks, and even wolves.

    Kaschmir, the Afghan Hound on the right, is a striking example of a dog that only dilutes phaeomelanin and not eumelanin. His mask is still very black, even though his body is almost white. For specific information on color testing in Afghans, please see their separate page.

    Photo by Barbara Lessmeister

    Another example of dilution of phaeomelanin only occurs in Tervuren. Buzz on the left demonstrates undilted red hairs, wheras Kosh on the right, has diluted red hairs. Both retain undiluted black hairs in their masks and intermingled on their bodies. Kosh would be called a gray by many Tervuren owners, quite understandably, but this further adds to the confusion around the term gray.


    Tyrosinase

    In many species, such as cats, rabbits, mice and cattle, there is a mutation that causes a temperature sensitive loss of pigmentation in the warm parts of the body. This phenotype is often called Himalayan and in most species the mutation that causes it was found in the tyrosinase gene. The dog at the left looks like the dog equivalent of a chocolate point Siamese cat. He was apparently found as a pup on the streets of Russia, but then disappeared again. It would have been interesting to study its tyrosinase sequence.

    To date no mutations affecting dog coat colors have been found in the coding sequence of Tyrosinase.


    The G Locus

    Little (1957) described graying as a progressive change resulting in a lightening of the hair coat as the dog ages. He suggested that this could be a dilution gene but it is not like the Weimaraner dilution gene, which causes pups to be born a diluted color and remain so. The gene that causes this in dogs, has not yet been discovered. Little (1957) reported that the G allele associated with greying is dominant.

    The gene that causes progessive graying has not yet been determined, as of 2020.

    This Standard Poodle demonstrates the effects of this gene in a very dramatic way. The small black spot by her neck is the result of a rabies vaccination! It will take a long time for that black hair to change to silver. Show dogs that have this coat color should be vaccinated in a less obvious place.

    Progressive graying also occurs in the Cairn Terrier, Bedlington Terrier, Havanese, Briard, Puli, Old English Sheepdog, Bearded Collie, Bouvier des Flanders and other breeds.

    Jester, the Kerry Blue Terrier at the right, illustrates that the muzzle of dogs with a EM allele will not not lighten by the same age as the rest of their body. Not all Kerry Blue Terrier lighten to the same extent.

    Linda Hall took the photos of some of her grey Puli over the years and has shared them. The photo above illustrates that Puli have "progressive greying". These dogs were all born black. She refers to them as "graying", "silver" and "black". Not all the Puli continue to go to the pale silver color. Some stay a darker grey.

    Progressive graying also occurs in horses and that trait was recently mapped to horse chromosome 25, considered the equivalent of human 9q. The gene has not yet been found.

  • Locke, M.M. et al. 2002. Linkage of the grey coat colour locus to microsatellites on horse chromosome 25. Animal Genetics 33:329-337
  • Swinburne J. E. et al. 2002. Assignment of the horse grey coat colour to ECA25 using whole genome scanning. Animal Genetics 33:338-342

  • Geriatric Graying

    Many dogs grey in advanced age. However most don't grey over all of their body. Contrary to progressive greying, geriatric greying is most noticable on the face. Egret, a Large Munsterlander, already had a white muzzle when young but the white around her eyes began about 10 or 11. The dark spot in her eye is another pigmentation change that can occur with age.

    The Labrador Retriever at the right has greyed mostly around the muzzle.


    Chinchilla Allele?

    Little discusses another hypothetical C allele, (cch). He uses this allele to explain both a pale brown and a pale red, which would imply it could dilute both eumelanin and phaeomelanin. He further states that phaeomelanin will be diluted before and more than eumelanin. In most species, both eumelanin and phaeomelanin are affected if TYR is mutated. However, MATP (now called SLC45A2) in horses has been shown to be a gene that affects the dilution of phaeomelanin and eumelanin differently by researchers in Davis, CA and in France. MATP mutations cause the "palomino/bucksin" colors in the horse. We have recently shown that this gene does not seem to cause dilutions to cream in several breeds of dogs. However, SLC45A2 is the gene associated with albinism in dogs, and thus is the "C" locus.

  • Schmutz, S. M., T. G. Berryere. 2007. The genetics of cream coat color in dogs. Journal of Heredity 98: 544-548.

  • Brown

    Some authors refer to brown as a dilute color. Somehow this bothers me. I don't think of brown as diluted black, which to me is gray or in dog terms "blue". Brown is created by a modification of eumelanin, so in some sense I suppose I can understand why it is sometimes lumped with the diluter gene effects.

    The gene for brown is TYRP1 and the 3 mutations causing brown are now known but this is discussed on another page about brown in this series.


    Other Dilution Genes - Not Yet Identified

    In several other species, more than one dilution gene has been identified. One of these is thought to be Tyrosinase Related Protein 2 (TRYP2). In mice, this gene dilutes black coat color to slaty grey and another name for the gene is therefore "slaty". Yet another name, based on it chemical composition, is DCT or Dopachrome tautomerase. This gene has been mapped to dog chromosome 22. In mice this DCT gene acts as a co-dominant I am unaware of a breed of dogs that has black, charcoal and pale grey so this gene may have no variants affecting coat color in dogs.

    Some poodle fanciers are concerned that the black in their breed is not as black as in breeds like the Labrador Retriever. I believe that may simply a side effect of the hair type that is characteristic of Poodles. However Poodles do have both the alleles for dilution and progressive graying and therefore many Poodles who might be black are blue or silver instead.

    A black poodle, Shadow, owned by Mrs. Ventner has become famous because it was the dog whose DNA was used by Celera to obtain the first "complete" dog sequence. IF there is something unique about poodle black.......Shadow would reveal that eventually. Shadow is 7 years old in the photo from Science magazine, September 26, 2003 issue.

    A larger effort to get a more detailed dog DNA sequence was conducted at NIH. A Boxer was used for the main dog in that study but about 9 other dogs of different breeds and a wolf was also partially sequenced. Much of this sequence is already available through GenBank.

    In the last few years, genome wide sequencing has become much more common and there are several labs that use this for research routinely.

  • Liu, P., S. H. Chen Y-W, Katz ML, Lubahn DB and Johnson GS. 1998. Length polymorphism in a CT-rich microsatellite in an intron of the canine tyrosinase-related protein-2 gene. Anim. Genet. 29:327.
  • Schmutz S.M., Moker J.S, Yuzbasiyan-Gurkan V., Zemke D., Sampson J., Lingaas F., Susana Dunner S., and G Dolf. 2001. DCT and EDNRB map to DogMap Linkage Group L07. Animal Genetics, in press

  • "Dilution" Loci

    Locus Symbol Gene Action Proven in Species Dog Chromosome
    D for dilute (Little) MLPH eumelanin diluted to grey or blue & phaeomelanin paled mouse, dog 25
    C for color (Little) SLC45A2 albinism in dogs, dilutes phaeomelanin & eumelanin horse 21
    P for pink-eyed (Little) P gene pink eyes & "white" coat mouse 5
    G for greying (Little) ??? progressive greying poodles and horse ?
    I for Intense (Iljin) MFSD12 only phaeomelanin diluted dog, horse 20
    Slaty TYRP2 eumelanin co-dominant dilution mouse 22

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